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ART1 ELISA Kit

This Mouse ART1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse ART1.
Catalog No. ABIN5651467

Quick Overview for ART1 ELISA Kit (ABIN5651467)

Target

See all ART1 ELISA Kits
ART1 (ADP-Ribosyltransferase 1 (ART1))

Reactivity

  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.781 ng/mL - 50 ng/mL

Application

ELISA

Sample Type

Tissue Homogenate
  • Minimum Detection Limit

    0.781 ng/mL

    Analytical Method

    Quantitative

    Specificity

    This assay has high sensitivity and excellent specificity for detection of ADP Ribosyltransferase 1 (ART1). No significant cross-reactivity or interference between ADP Ribosyltransferase 1 (ART1) and analogues was observed.

    Sensitivity

    0.32 ng/mL
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time

    3 h

    Plate

    Pre-coated

    Protocol

    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADP Ribosyltransferase 1 (ART1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ADP Ribosyltransferase 1 (ART1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADP Ribosyltransferase 1 (ART1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADP Ribosyltransferase 1 (ART1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Assay Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADP Ribosyltransferase 1 (ART1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADP Ribosyltransferase 1 (ART1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Handling Advice

    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

    Storage

    4 °C,-20 °C

    Storage Comment

    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

    Expiry Date

    4-8 months
  • Target See all ART1 ELISA Kits

    ART1 (ADP-Ribosyltransferase 1 (ART1))

    Alternative Name

    ADP Ribosyltransferase 1

    Background

    Gene Name: ADP Ribosyltransferase 1

    Gene Aliases: CD296, ART2, ARTC1, Mono(ADP-ribosyl)transferase 1, ADP-ribosyltransferase C2 and C3 toxin-like 1, GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1

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