ALDH1A2 ELISA Kit

Catalog No. ABIN5651558

Product Details ALDH1A2 ELISA Kit

Target: ALDH1A2 (Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2). No significant cross-reactivity or interference between Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) and analogues was observed.

Sensitivity: 0.215 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ALDH1A2

Target: ALDH1A2 (Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2))

Alternative Name: Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2 Products)

Synonyms: Raldh-2 ELISA Kit, RALDH(II) ELISA Kit, RALDH2 ELISA Kit, RALDH2-T ELISA Kit, fb50h01 ELISA Kit, raldh2 ELISA Kit, AV116159 ELISA Kit, Aldh1a7 ELISA Kit, Raldh1 ELISA Kit, Raldh2 ELISA Kit, aldehyde dehydrogenase 1 family, member A2 ELISA Kit, aldehyde dehydrogenase 1 family member A2 ELISA Kit, aldehyde dehydrogenase family 1, subfamily A2 ELISA Kit, Aldh1a2 ELISA Kit, ALDH1A2 ELISA Kit, aldh1a2 ELISA Kit

Background:

Gene Name: Aldehyde Dehydrogenase 1 Family, Member A2

Gene Aliases: RALDH(II), RALDH2, RALDH2-T, Retinaldehyde Dehydrogenase 2, Retinal dehydrogenase 2, Retinaldehyde-specific dehydrogenase type 2

Gene ID: 8854

UniProt: O94788

Pathways: Retinoic Acid Receptor Signaling Pathway