alpha 2 Macroglobulin CLIA Kit

Catalog No. ABIN5651629

Product Details alpha 2 Macroglobulin CLIA Kit

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.39 ng/mL - 100 ng/mL

Minimum Detection Limit: 0.39 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Urine

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin (a2M). No significant cross-reactivity or interference between Alpha-2-Macroglobulin (a2M) and analogues was observed.

Sensitivity: 0.05 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin (a2M). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin (a2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alpha-2-Macroglobulin (a2M) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: Alpha-2-Macroglobulin (A2M Products)

Synonyms: A2MD CLIA Kit, CPAMD5 CLIA Kit, FWP007 CLIA Kit, S863-7 CLIA Kit, A2MAC1 CLIA Kit, A2m1 CLIA Kit, A2maa CLIA Kit, Mam CLIA Kit, A2mp CLIA Kit, A2M CLIA Kit, LOC733429 CLIA Kit, endod CLIA Kit, cpamd5 CLIA Kit, fwp007 CLIA Kit, s863-7 CLIA Kit, a2mb CLIA Kit, endodermin CLIA Kit, Alpha-2-M CLIA Kit, alpha-2-macroglobulin CLIA Kit, pregnancy-zone protein L homeolog CLIA Kit, alpha-2-macroglobulin S homeolog CLIA Kit, pregnancy zone protein CLIA Kit, alpha-2-macroglobulin-like CLIA Kit, A2M CLIA Kit, A2m CLIA Kit, LOC477699 CLIA Kit, pzp.L CLIA Kit, AZL_c00450 CLIA Kit, LOC100349077 CLIA Kit, a2m CLIA Kit, LOC100061656 CLIA Kit, LOC100090399 CLIA Kit, a2m.S CLIA Kit, LOC100469973 CLIA Kit, LOC100595735 CLIA Kit, PZP CLIA Kit, LOC100353095 CLIA Kit, LOC101122940 CLIA Kit, LOC101801552 CLIA Kit, LOC100911545 CLIA Kit

Background:

Gene Name: Alpha-2-Macroglobulin

Gene Aliases: A2MG, A2 mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Gene ID: 2

UniProt: P01023

Pathways: Lipid Metabolism