Vitamin E CLIA Kit

Catalog No. ABIN5651659

The Various Species Vitamin E ELISA Kit (ABIN5651659) is a Chemiluminescent ELISA Kit designed to quantify Various Species Vitamin E.

Quick Overview for Vitamin E CLIA Kit (ABIN5651659)

Target: Vitamin E

Reactivity: Various Species

Detection Method: Chemiluminescent

Method Type: Competition ELISA

Detection Range: 781.25 ng/mL - 200000 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Vitamin E CLIA Kit

Minimum Detection Limit: 781.25 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Alpha-Tocopherol (TCPa). No significant cross-reactivity or interference between Alpha-Tocopherol (TCPa) and analogues was observed.

Sensitivity: 271 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Alpha-Tocopherol (TCPa). A competitive inhibition reaction is launched between biotin labeled Alpha-Tocopherol (TCPa) and unlabeled Alpha-Tocopherol (TCPa) (Standards or samples) with the pre-coated antibody specific to Alpha-Tocopherol (TCPa). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Tocopherol (TCPa) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Alpha-Tocopherol (TCPa) level in the sample or standard.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Tocopherol (TCPa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Tocopherol (TCPa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Vitamin E

Alternative Name: Alpha-Tocopherol

Target Type: Chemical

Background:

Gene Name: Alpha-Tocopherol

Gene Aliases: VE, Vitamin E, α-TCP, α-Tocopherol