Annexin a1 ELISA Kit

Catalog No. ABIN5651825

Product Details Annexin a1 ELISA Kit

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Annexin A1 (ANXA1). No significant cross-reactivity or interference between Annexin A1 (ANXA1) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin A1 (ANXA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Annexin A1 (ANXA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: Annexin A1 (ANXA1 Products)

Synonyms: anx1a ELISA Kit, anxa1 ELISA Kit, wu:fa01d11 ELISA Kit, wu:fa05d11 ELISA Kit, wu:fk69h01 ELISA Kit, MGC64363 ELISA Kit, anx1 ELISA Kit, lpc1 ELISA Kit, MGC89164 ELISA Kit, ANXA1 ELISA Kit, ANX1 ELISA Kit, LPC1 ELISA Kit, p35 ELISA Kit, Anx-1 ELISA Kit, Anx-A1 ELISA Kit, C430014K04Rik ELISA Kit, Lpc-1 ELISA Kit, Lpc1 ELISA Kit, Anx1 ELISA Kit, annexin A1a ELISA Kit, annexin A1 ELISA Kit, annexin A1 L homeolog ELISA Kit, Annexin A1 ELISA Kit, anxa1a ELISA Kit, anxa1 ELISA Kit, anxa1.L ELISA Kit, ANXA1 ELISA Kit, LOC100335033 ELISA Kit, Anxa1 ELISA Kit

Background:

Gene Name: Annexin A1

Gene Aliases: ANX-A1, ANX1, LPC1, Lipocortin I, Chromobindin-9, Calpactin II, Phospholipase A2 inhibitory protein

Gene ID: 301

UniProt: P04083

Pathways: Hormone Transport