AGR2 CLIA Kit

Catalog No. ABIN5651846

Human AGR2 ELISA Kit, Chemiluminescent assay for quantification of Human AGR2.

Quick Overview for AGR2 CLIA Kit (ABIN5651846)

Target: AGR2 (Anterior Gradient Homolog 2 (Xenopus Laevis) (AGR2))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 13.7 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details AGR2 CLIA Kit

Minimum Detection Limit: 13.7 pg/mL

Purpose: Human Anterior Gradient 2 (AGR2) CLIA Kit for detecting AGR2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Anterior Gradient Protein 2 (AGR2). No significant cross-reactivity or interference between Anterior Gradient Protein 2 (AGR2) and analogues was observed.

Sensitivity: 5.5 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Anterior Gradient Protein 2 (AGR2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Anterior Gradient Protein 2 (AGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Anterior Gradient Protein 2 (AGR2) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anterior Gradient Protein 2 (AGR2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anterior Gradient Protein 2 (AGR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: AGR2 (Anterior Gradient Homolog 2 (Xenopus Laevis) (AGR2))

Alternative Name: Anterior Gradient 2

Background: Gene Aliases: GOB4, HAG2, XAG2, AG2, PDIA17, HPC8, Anterior Gradient Protein 2, Protein Disulfide Isomerase Family A,Member 17, Secreted cement gland protein XAG-2 homolog

Gene ID: 10551

UniProt: O95994