AIF ELISA Kit

Catalog No. ABIN5651957

Product Details AIF ELISA Kit

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Apoptosis Inducing Factor (AIF). No significant cross-reactivity or interference between Apoptosis Inducing Factor (AIF) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptosis Inducing Factor (AIF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apoptosis Inducing Factor (AIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptosis Inducing Factor (AIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apoptosis Inducing Factor (AIF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apoptosis Inducing Factor (AIF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apoptosis Inducing Factor (AIF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: Apoptosis Inducing Factor (AIFM1 Products)

Synonyms: AIF ELISA Kit, CMTX4 ELISA Kit, COWCK ELISA Kit, COXPD6 ELISA Kit, PDCD8 ELISA Kit, CG7263 ELISA Kit, DmAIF ELISA Kit, Dmel\\CG7263 ELISA Kit, GB16024 ELISA Kit, DDBDRAFT_0187853 ELISA Kit, DDBDRAFT_0191137 ELISA Kit, DDB_0187853 ELISA Kit, DDB_0191137 ELISA Kit, aif ELISA Kit, pdcd8 ELISA Kit, AIFM1 ELISA Kit, PCD8 ELISA Kit, AIFsh2 ELISA Kit, Hq ELISA Kit, Pdcd8 ELISA Kit, mAIF ELISA Kit, Aif ELISA Kit, zgc:91994 ELISA Kit, apoptosis inducing factor mitochondria associated 1 ELISA Kit, allograft inflammatory factor 1 ELISA Kit, Apoptosis inducing factor ELISA Kit, apoptosis-inducing factor 1, mitochondrial ELISA Kit, apoptosis inducing factor ELISA Kit, apoptosis inducing factor, mitochondria associated 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated, 1 ELISA Kit, apoptosis-inducing factor, mitochondrion-associated 1 ELISA Kit, AIFM1 ELISA Kit, AIF1 ELISA Kit, AIF ELISA Kit, LOC412212 ELISA Kit, aif ELISA Kit, aifm1 ELISA Kit, Aifm1 ELISA Kit

Background:

Gene Name: Apoptosis Inducing Factor

Gene Aliases: PDCD8, Programmed Cell Death protein 8, Apoptosis-inducing factor 1, mitochondrial

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect