beta Arrestin 1 ELISA Kit

Catalog No. ABIN5652019

Product Details beta Arrestin 1 ELISA Kit

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Arrestin Beta 1 (ARRb1). No significant cross-reactivity or interference between Arrestin Beta 1 (ARRb1) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arrestin Beta 1 (ARRb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arrestin Beta 1 (ARRb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arrestin Beta 1 (ARRb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arrestin Beta 1 (ARRb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arrestin Beta 1 (ARRb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arrestin Beta 1 (ARRb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for beta Arrestin 1

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Alternative Name: Arrestin Beta 1 (ARRB1 Products)

Synonyms: arrb1 ELISA Kit, MGC83787 ELISA Kit, arb1 ELISA Kit, arr1 ELISA Kit, barr1 ELISA Kit, betaarr1 ELISA Kit, 1200006I17Rik ELISA Kit, AW208571 ELISA Kit, G430100A01Rik ELISA Kit, ARB1 ELISA Kit, ARR1 ELISA Kit, BARRES ELISA Kit, arrestin beta 1 ELISA Kit, arrestin, beta 1 ELISA Kit, arrestin beta 1 L homeolog ELISA Kit, beta-arrestin 1 ELISA Kit, putative beta-arrestin 1 ELISA Kit, ARRB1 ELISA Kit, arrb1 ELISA Kit, arrb1.L ELISA Kit, CpipJ_CPIJ016988 ELISA Kit, CpipJ_CPIJ017114 ELISA Kit, Smp_122900 ELISA Kit, Smp_126080 ELISA Kit, Smp_176980 ELISA Kit, Smp_152290 ELISA Kit, Arrb1 ELISA Kit

Background:

Gene Name: Arrestin Beta 1

Gene Aliases: ARB1, ARR1, ARR-B1, Arrestin 2

Pathways: Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction