Aryl Hydrocarbon Receptor CLIA Kit

Catalog No. ABIN5652023

Product Details Aryl Hydrocarbon Receptor CLIA Kit

Target: Aryl Hydrocarbon Receptor (AHR)

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 39.06 pg/mL - 10000 pg/mL

Minimum Detection Limit: 39.06 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aryl Hydrocarbon Receptor (AhR). No significant cross-reactivity or interference between Aryl Hydrocarbon Receptor (AhR) and analogues was observed.

Sensitivity: 5.87 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor (AhR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor (AhR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Aryl Hydrocarbon Receptor (AhR) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor (AhR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor (AhR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Aryl Hydrocarbon Receptor (AHR)

Alternative Name: Aryl Hydrocarbon Receptor (AHR Products)

Synonyms: AHR CLIA Kit, ahr1 CLIA Kit, ahr CLIA Kit, bHLHe76 CLIA Kit, Ah CLIA Kit, Ahh CLIA Kit, Ahre CLIA Kit, In CLIA Kit, aryl hydrocarbon receptor S homeolog CLIA Kit, aryl hydrocarbon receptor CLIA Kit, aryl-hydrocarbon receptor CLIA Kit, ahr.S CLIA Kit, AHR CLIA Kit, ahr CLIA Kit, Ahr CLIA Kit

Background:

Gene Name: Aryl Hydrocarbon Receptor

Gene Aliases: BHLHE76, Class E basic helix-loop-helix protein 76

Gene ID: 196

UniProt: P35869

Pathways: Regulation of Cell Size