ARNT ELISA Kit

Catalog No. ABIN5652026

Product Details ARNT ELISA Kit

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). No significant cross-reactivity or interference between Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT Products)

Synonyms: HIF-1-beta ELISA Kit, HIF-1beta ELISA Kit, HIF1-beta ELISA Kit, HIF1B ELISA Kit, HIF1BETA ELISA Kit, TANGO ELISA Kit, bHLHe2 ELISA Kit, D3Ertd557e ELISA Kit, Drnt ELISA Kit, ESTM42 ELISA Kit, Hif1b ELISA Kit, W08714 ELISA Kit, mKIAA4051 ELISA Kit, Arnt1 ELISA Kit, ARNT ELISA Kit, zgc:136664 ELISA Kit, arnt1 ELISA Kit, hif1b ELISA Kit, tango ELISA Kit, xArnt ELISA Kit, hif1beta ELISA Kit, hif-1beta ELISA Kit, arnt.2 ELISA Kit, MGC68805 ELISA Kit, aryl hydrocarbon receptor nuclear translocator ELISA Kit, aryl hydrocarbon receptor nuclear translocator S homeolog ELISA Kit, ARNT ELISA Kit, Arnt ELISA Kit, arnt ELISA Kit, arnt.S ELISA Kit

Background:

Gene Name: Aryl Hydrocarbon Receptor Nuclear Translocator

Gene Aliases: HIF-1beta, bHLHe2, Hypoxia Inducible Factor 1 Beta, Class E basic helix-loop-helix protein 2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta

Gene ID: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect