GOT1 ELISA Kit

Catalog No. ABIN5652044

Product Details GOT1 ELISA Kit

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Aspartate Aminotransferase (AST). No significant cross-reactivity or interference between Aspartate Aminotransferase (AST) and analogues was observed.

Sensitivity: 0.114 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Aminotransferase (AST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Aminotransferase (AST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Aminotransferase (AST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Aminotransferase (AST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: Aspartate Aminotransferase (GOT1 Products)

Synonyms: ASTQTL1 ELISA Kit, GIG18 ELISA Kit, cAspAT ELISA Kit, cCAT ELISA Kit, AST ELISA Kit, ISSD ELISA Kit, NSD ELISA Kit, SD ELISA Kit, SIALIN ELISA Kit, SIASD ELISA Kit, SLD ELISA Kit, xr406 ELISA Kit, CASPAT ELISA Kit, ASPARTATE AMINOTRANSFERASE ELISA Kit, ATAAT ELISA Kit, MATERNAL EFFECT EMBRYO ARREST 17 ELISA Kit, MEE17 ELISA Kit, T26C19.9 ELISA Kit, T26C19_9 ELISA Kit, aspartate aminotransferase ELISA Kit, aatA ELISA Kit, 83.t00021 ELISA Kit, AI789014 ELISA Kit, Got-1 ELISA Kit, Aspat ELISA Kit, Gaspat ELISA Kit, AL022787 ELISA Kit, FABP-pm ELISA Kit, Got-2 ELISA Kit, mAspAT ELISA Kit, ASPATA ELISA Kit, glutamic-oxaloacetic transaminase 1 ELISA Kit, solute carrier family 17 member 5 ELISA Kit, glutamic-oxaloacetic transaminase 1 homeolog ELISA Kit, aspartate aminotransferase ELISA Kit, glutamic-oxaloacetic transaminase 1, soluble ELISA Kit, glutamic-oxaloacetic transaminase 2 ELISA Kit, glutamatic-oxaloacetic transaminase 2, mitochondrial ELISA Kit, GOT1 ELISA Kit, SLC17A5 ELISA Kit, got1.S ELISA Kit, AAT ELISA Kit, aspC ELISA Kit, EHI_006080 ELISA Kit, AST2 ELISA Kit, ast2 ELISA Kit, got1 ELISA Kit, Got1 ELISA Kit, GOT2 ELISA Kit, Got2 ELISA Kit

Background:

Gene Name: Aspartate Aminotransferase

Gene Aliases: cCAT, AAT, ASAT, SGOT, GOT1, Cysteine transaminase, cytoplasmic, Transaminase A, Aspartate Transaminase 1,Cytoplasmic, Glutamic-Oxaloacetic Transaminase 1,Soluble

UniProt: P05201

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process