ATG12 ELISA Kit

Catalog No. ABIN5652118

Product Details ATG12 ELISA Kit

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 12 (ATG12). No significant cross-reactivity or interference between Autophagy Related Protein 12 (ATG12) and analogues was observed.

Sensitivity: 13.1 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 12 (ATG12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 12 (ATG12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 12 (ATG12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 12 (ATG12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 12 (ATG12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 12 (ATG12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: Autophagy Related Protein 12 (ATG12 Products)

Synonyms: APG12 ELISA Kit, APG12L ELISA Kit, FBR93 ELISA Kit, HAPG12 ELISA Kit, Apg12l ELISA Kit, AUTOPHAGY 12 ELISA Kit, AUTOPHAGY 12 B ELISA Kit, CG10861 ELISA Kit, Dmel\\CG10861 ELISA Kit, atg12 ELISA Kit, ATG12 ELISA Kit, apg12 ELISA Kit, DDBDRAFT_0185323 ELISA Kit, DDBDRAFT_0191412 ELISA Kit, DDB_0185323 ELISA Kit, DDB_0191412 ELISA Kit, 4931423H11Rik ELISA Kit, A330058M13Rik ELISA Kit, Atg12l ELISA Kit, AO090001000593 ELISA Kit, autophagy related 12 ELISA Kit, Ubiquitin-like superfamily protein ELISA Kit, Atg12p ELISA Kit, autophagy protein ELISA Kit, ubiquitin-like protein ATG12B ELISA Kit, autophagy associated protein Atg12 ELISA Kit, Autophagy-related 12 ELISA Kit, ATG12 autophagy related 12 homolog (S. cerevisiae) ELISA Kit, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene ELISA Kit, autophagy associated ubiquitin-like modifier Atg12 ELISA Kit, hypothetical protein ELISA Kit, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) ELISA Kit, autophagy protein 12 ELISA Kit, autophagy related 12 S homeolog ELISA Kit, autophagy-related protein 12 ELISA Kit, ATG12 ELISA Kit, Atg12 ELISA Kit, APG12B ELISA Kit, APG12 ELISA Kit, LOC9321066 ELISA Kit, apg12 ELISA Kit, atg12 ELISA Kit, LOC743425 ELISA Kit, CAALFM_C113950CA ELISA Kit, atg12.S ELISA Kit, ANI_1_922094 ELISA Kit, AOR_1_1064164 ELISA Kit

Background:

Gene Name: Autophagy Related Protein 12

Gene Aliases: APG12, APG12L, HAPG12, Ubiquitin-like protein ATG12

Pathways: Autophagy