BTK ELISA Kit

Catalog No. ABIN5652356

Product Details BTK ELISA Kit

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Bruton'S Tyrosine Kinase (Btk). No significant cross-reactivity or interference between Bruton'S Tyrosine Kinase (Btk) and analogues was observed.

Sensitivity: 0.06 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bruton'S Tyrosine Kinase (Btk). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bruton'S Tyrosine Kinase (Btk). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bruton'S Tyrosine Kinase (Btk), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bruton'S Tyrosine Kinase (Btk) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bruton'S Tyrosine Kinase (Btk) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bruton'S Tyrosine Kinase (Btk) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: Bruton'S Tyrosine Kinase (BTK Products)

Synonyms: BTK ELISA Kit, atk ELISA Kit, bpk ELISA Kit, xla ELISA Kit, imd1 ELISA Kit, agmx1 ELISA Kit, psctk1 ELISA Kit, AGMX1 ELISA Kit, AT ELISA Kit, ATK ELISA Kit, BPK ELISA Kit, IMD1 ELISA Kit, PSCTK1 ELISA Kit, XLA ELISA Kit, AI528679 ELISA Kit, xid ELISA Kit, Bruton tyrosine kinase ELISA Kit, Bruton agammaglobulinemia tyrosine kinase ELISA Kit, BTK ELISA Kit, btk ELISA Kit, Btk ELISA Kit

Background:

Gene Name: Bruton'S Tyrosine Kinase

Gene Aliases: AGMX1, AT, ATK, BPK, IMD1, PSCTK1, XLA, Bruton Agammaglobulinemia Tyrosine Kinase, B-cell progenitor kinase

UniProt: E9PTF7

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling