CACYBP ELISA Kit

Catalog No. ABIN5652501

Product Details CACYBP ELISA Kit

Target: CACYBP (Calcyclin Binding Protein (CACYBP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Calcyclin Binding Protein (CACYBP). No significant cross-reactivity or interference between Calcyclin Binding Protein (CACYBP) and analogues was observed.

Sensitivity: 33 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcyclin Binding Protein (CACYBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcyclin Binding Protein (CACYBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcyclin Binding Protein (CACYBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcyclin Binding Protein (CACYBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcyclin Binding Protein (CACYBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcyclin Binding Protein (CACYBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CACYBP

Target: CACYBP (Calcyclin Binding Protein (CACYBP))

Alternative Name: Calcyclin Binding Protein (CACYBP Products)

Synonyms: zgc:76993 ELISA Kit, CACYBP ELISA Kit, sip ELISA Kit, gig5 ELISA Kit, pnas-107 ELISA Kit, s100a6bp ELISA Kit, T1P2.12 ELISA Kit, T1P2_12 ELISA Kit, GIG5 ELISA Kit, RP1-102G20.6 ELISA Kit, S100A6BP ELISA Kit, SIP ELISA Kit, calcyclin binding protein ELISA Kit, calcyclin binding protein, putative ELISA Kit, Calcyclin binding protein, putative ELISA Kit, SGS domain-containing protein ELISA Kit, calcyclin binding protein L homeolog ELISA Kit, cacybp ELISA Kit, CACYBP ELISA Kit, PB000926.02.0 ELISA Kit, PCHAS_145490 ELISA Kit, PVX_100855 ELISA Kit, PKH_145680 ELISA Kit, AT1G30070 ELISA Kit, cacybp.L ELISA Kit, Cacybp ELISA Kit

Background:

Gene Name: Calcyclin Binding Protein

Gene Aliases: SIP, GIG5, PNAS-107, RP1-102G20.6, S100A6BP, S100A6-binding protein, Siah-interacting protein

Gene ID: 27101

UniProt: Q9HB71

Pathways: Response to Growth Hormone Stimulus