Calreticulin ELISA Kit

Catalog No. ABIN5652525

Product Details Calreticulin ELISA Kit

Target: Calreticulin (CALR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Calreticulin (CRT). No significant cross-reactivity or interference between Calreticulin (CRT) and analogues was observed.

Sensitivity: 0.273 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calreticulin (CRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calreticulin (CRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calreticulin (CRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calreticulin (CRT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calreticulin (CRT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: Calreticulin (CALR Products)

Synonyms: CRT ELISA Kit, RO ELISA Kit, SSA ELISA Kit, cC1qR ELISA Kit, crc ELISA Kit, crt ELISA Kit, Calregulin ELISA Kit, CG9429 ELISA Kit, Clr ELISA Kit, Dmel\\CG9429 ELISA Kit, anon-EST:Liang-2.11 ELISA Kit, anon-EST:Liang-2.17 ELISA Kit, anon-EST:fe3D7 ELISA Kit, clone 2.11 ELISA Kit, clone 2.17 ELISA Kit, eth ELISA Kit, potp ELISA Kit, cc1qr ELISA Kit, ssa ELISA Kit, NV19007 ELISA Kit, CRP55 ELISA Kit, ERp60 ELISA Kit, HACBP ELISA Kit, CHOT1 ELISA Kit, CHT1 ELISA Kit, CT1 ELISA Kit, AA589632 ELISA Kit, CRTR ELISA Kit, Creat ELISA Kit, CCDS1 ELISA Kit, calrl2 ELISA Kit, fc66d10 ELISA Kit, wu:fc66d10 ELISA Kit, wu:fj50c03 ELISA Kit, zgc:66153 ELISA Kit, calreticulin ELISA Kit, calreticulin L homeolog ELISA Kit, Calreticulin ELISA Kit, putative calreticulin ELISA Kit, solute carrier family 6 member 8 ELISA Kit, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 ELISA Kit, CALR ELISA Kit, calr.L ELISA Kit, Crt ELISA Kit, crt ELISA Kit, CRT ELISA Kit, Calr ELISA Kit, calr ELISA Kit, LMJF_31_2600 ELISA Kit, Crc ELISA Kit, Slc6a8 ELISA Kit, SLC6A8 ELISA Kit, LOC8269812 ELISA Kit

Background:

Gene Name: Calreticulin

Gene Aliases: CALR, RO, SSA, CC1qR, ERp60, HACBP, grp60, CRTC, CRP55, Calregulin, Sicca Syndrome Antigen A, Autoantigen Ro, Endoplasmic reticulum resident protein 60

Gene ID: 811

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response