Carboxy Methyl Lysine ELISA Kit

Catalog No. ABIN5652567

This Colorimetric ELISA kit is designed for the quantitative measurement of Various Species Carboxy Methyl Lysine.

Quick Overview for Carboxy Methyl Lysine ELISA Kit (ABIN5652567)

Target: Carboxy Methyl Lysine (CML)

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 61.7 ng/mL - 5000 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Carboxy Methyl Lysine ELISA Kit

Minimum Detection Limit: 61.7 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Carboxymethyl Lysine (CML). No significant cross-reactivity or interference between Carboxymethyl Lysine (CML) and analogues was observed.

Sensitivity: 25.9 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carboxymethyl Lysine (CML) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carboxymethyl Lysine (CML) and unlabeled Carboxymethyl Lysine (CML) (Standards or samples) with the pre-coated antibody specific to Carboxymethyl Lysine (CML). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carboxymethyl Lysine (CML) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carboxymethyl Lysine (CML) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxymethyl Lysine (CML) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxymethyl Lysine (CML) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Carboxy Methyl Lysine

Target: Carboxy Methyl Lysine (CML)

Alternative Name: Carboxymethyl Lysine

Target Type: Amino Acid

Background:

Gene Name: Carboxymethyl Lysine

Gene Aliases: N(6)-Carboxymethyllysine