CPB1 ELISA Kit

Catalog No. ABIN5652574

Product Details CPB1 ELISA Kit

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6.25 pg/mL - 400 pg/mL

Minimum Detection Limit: 6.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Carboxypeptidase B1, Tissue (CPB1). No significant cross-reactivity or interference between Carboxypeptidase B1, Tissue (CPB1) and analogues was observed.

Sensitivity: 2.98 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase B1, Tissue (CPB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase B1, Tissue (CPB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase B1, Tissue (CPB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxypeptidase B1, Tissue (CPB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxypeptidase B1, Tissue (CPB1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxypeptidase B1, Tissue (CPB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CPB1

Target: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Alternative Name: Carboxypeptidase B1, Tissue (CPB1 Products)

Synonyms: wu:fb60d07 ELISA Kit, wu:fb69b08 ELISA Kit, CPB1 ELISA Kit, pCPB ELISA Kit, CPB ELISA Kit, PASP ELISA Kit, PCPB ELISA Kit, Cpb ELISA Kit, 0910001A18Rik ELISA Kit, 1810063F02Rik ELISA Kit, 2210008M23Rik ELISA Kit, AI504870 ELISA Kit, carboxypeptidase B1 (tissue) ELISA Kit, carboxypeptidase B1 ELISA Kit, carboxypeptidase B1 S homeolog ELISA Kit, carboxypeptidase B ELISA Kit, cpb1 ELISA Kit, CPB1 ELISA Kit, cpb1.S ELISA Kit, CpipJ_CPIJ010799 ELISA Kit, CpipJ_CPIJ010801 ELISA Kit, VDBG_02663 ELISA Kit, LOC100562609 ELISA Kit, Cpb1 ELISA Kit

Background:

Gene Name: Carboxypeptidase B1, Tissue

Gene Aliases: CPB, PCPB, PASP, Pancreas-specific protein

Gene ID: 76703

UniProt: B2RS76