Catalase ELISA Kit

Catalog No. ABIN5652674

Product Details Catalase ELISA Kit

Target: Catalase (CAT)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Catalase (CAT). No significant cross-reactivity or interference between Catalase (CAT) and analogues was observed.

Sensitivity: 0.33 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catalase (CAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catalase (CAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catalase (CAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catalase (CAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catalase (CAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catalase (CAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: Catalase (CAT Products)

Synonyms: 2210418N07 ELISA Kit, Cas-1 ELISA Kit, Cas1 ELISA Kit, Cs-1 ELISA Kit, CS1 ELISA Kit, Cat01 ELISA Kit, Catl ELISA Kit, fb68a12 ELISA Kit, wu:fb68a12 ELISA Kit, CAT ELISA Kit, CATA ELISA Kit, CG6871 ELISA Kit, CT21282 ELISA Kit, CatA ELISA Kit, DMCATHPO ELISA Kit, DROCATHPO ELISA Kit, Dmel\\CG6871 ELISA Kit, U00145 ELISA Kit, bs36h11.y1 ELISA Kit, cat ELISA Kit, GB11648 ELISA Kit, catalase ELISA Kit, Cat ELISA Kit, BA0843 ELISA Kit, DKFZp469E0232 ELISA Kit, catalase ELISA Kit, Catalase ELISA Kit, catalase, gene 2 ELISA Kit, CAT ELISA Kit, Cat ELISA Kit, cat ELISA Kit, cat.2 ELISA Kit, LOC769804 ELISA Kit, BA_0843 ELISA Kit

Background:

Gene Name: Catalase

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism