Cathepsin D ELISA Kit

Catalog No. ABIN5652695

Product Details Cathepsin D ELISA Kit

Target: Cathepsin D (CTSD)

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 2.47 ng/mL - 200 ng/mL

Minimum Detection Limit: 2.47 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin D (CTSD). No significant cross-reactivity or interference between Cathepsin D (CTSD) and analogues was observed.

Sensitivity: 0.88 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cathepsin D (CTSD) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cathepsin D (CTSD) and unlabeled Cathepsin D (CTSD) (Standards or samples) with the pre-coated antibody specific to Cathepsin D (CTSD). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cathepsin D (CTSD) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cathepsin D (CTSD) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D (CTSD Products)

Synonyms: catD ELISA Kit, fb93e11 ELISA Kit, fj17b09 ELISA Kit, wu:fb93e11 ELISA Kit, wu:fj17b09 ELISA Kit, LOC398557 ELISA Kit, Cathd ELISA Kit, CatD ELISA Kit, LOC100136761 ELISA Kit, CTSD ELISA Kit, DKFZp469J0315 ELISA Kit, DDBDRAFT_0205785 ELISA Kit, DDBDRAFT_0215012 ELISA Kit, DDB_0205785 ELISA Kit, DDB_0215012 ELISA Kit, CLN10 ELISA Kit, CPSD ELISA Kit, CD ELISA Kit, cathepsin D ELISA Kit, cathepsin D S homeolog ELISA Kit, lysosomal aspartic protease ELISA Kit, cathepsin D-like ELISA Kit, ctsd ELISA Kit, LOC398557 ELISA Kit, ctsd.S ELISA Kit, CatD ELISA Kit, LOC5567565 ELISA Kit, LOC100136761 ELISA Kit, CTSD ELISA Kit, Cathd ELISA Kit, ctsD ELISA Kit, catd ELISA Kit, Ctsd ELISA Kit, LOC443060 ELISA Kit

Background:

Gene Name: Cathepsin D

Gene Aliases: CPSD, CLN10, Lysosomal Aspartyl Protease, Ceroid-Lipofuscinosis,Neuronal 10

Pathways: Peptide Hormone Metabolism