Cholecystokinin ELISA Kit

Catalog No. ABIN5652880

The Human Cholecystokinin ELISA Kit (ABIN5652880) is a Colorimetric ELISA Kit designed to quantify Human Cholecystokinin.

Quick Overview for Cholecystokinin ELISA Kit (ABIN5652880)

Target: Cholecystokinin (CCK)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 12.35 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Cholecystokinin ELISA Kit

Minimum Detection Limit: 12.35 pg/mL

Purpose: Human Cholecystokinin 12 (CCK12) ELISA Kit for detecting CCK12 in serum, plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cholecystokinin 12 (CCK12). No significant cross-reactivity or interference between Cholecystokinin 12 (CCK12) and analogues was observed.

Sensitivity: 4.15 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 50 μL

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin 12 (CCK12) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin 12 (CCK12) and unlabeled Cholecystokinin 12 (CCK12) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin 12 (CCK12). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin 12 (CCK12) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin 12 (CCK12) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin 12 (CCK12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin 12 (CCK12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details for Cholecystokinin

Target: Cholecystokinin (CCK)

Alternative Name: Cholecystokinin 12

Background: Gene Aliases:

Gene ID: 885

UniProt: P06307

Pathways: TCR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades, Feeding Behaviour