Factor VII ELISA Kit

Catalog No. ABIN5653051

Product Details Factor VII ELISA Kit

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VII (F7). No significant cross-reactivity or interference between Coagulation Factor VII (F7) and analogues was observed.

Sensitivity: 12.4 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VII (F7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor VII (F7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VII (F7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor VII (F7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor VII (F7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor VII (F7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Factor VII

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Alternative Name: Coagulation Factor VII (F7 Products)

Synonyms: F7 ELISA Kit, wu:fb59f04 ELISA Kit, zgc:109870 ELISA Kit, SPCA ELISA Kit, AI114908 ELISA Kit, ATIII ELISA Kit, At-3 ELISA Kit, At3 ELISA Kit, LOC100223776 ELISA Kit, AI132620 ELISA Kit, Cf7 ELISA Kit, FVII ELISA Kit, mfVII ELISA Kit, f7 ELISA Kit, coagulation factor VII ELISA Kit, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 ELISA Kit, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog ELISA Kit, F7 ELISA Kit, f7 ELISA Kit, Serpinc1 ELISA Kit, CpipJ_CPIJ009142 ELISA Kit, CpipJ_CPIJ010295 ELISA Kit, CpipJ_CPIJ020127 ELISA Kit, fa7 ELISA Kit, f7.S ELISA Kit

Background:

Gene Name: Coagulation Factor VII

Gene Aliases: FVII, SPCA, proconvertin, Cothromboplastin, Serum Prothrombin Conversation Accelerator, Stable Factor, Eptacog alfa

Gene ID: 260320

UniProt: Q8K3U6

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling