Factor XI ELISA Kit

Catalog No. ABIN5653062

Product Details Factor XI ELISA Kit

Target: Factor XI (F11) (Coagulation Factor XI (F11))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.47 ng/mL - 30 ng/mL

Minimum Detection Limit: 0.47 ng/mL

Application: ELISA

Sample Type: Plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor XI (F11). No significant cross-reactivity or interference between Coagulation Factor XI (F11) and analogues was observed.

Sensitivity: 0.21 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XI (F11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor XI (F11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor XI (F11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor XI (F11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor XI (F11) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor XI (F11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Factor XI

Target: Factor XI (F11) (Coagulation Factor XI (F11))

Alternative Name: Coagulation Factor XI (F11 Products)

Synonyms: FXI ELISA Kit, 1600027G01Rik ELISA Kit, AI503996 ELISA Kit, Cf11 ELISA Kit, LRRGT00086 ELISA Kit, coagulation factor XI ELISA Kit, F11 ELISA Kit, CpipJ_CPIJ000593 ELISA Kit, CpipJ_CPIJ001060 ELISA Kit, CpipJ_CPIJ002752 ELISA Kit, CpipJ_CPIJ003631 ELISA Kit, CpipJ_CPIJ004041 ELISA Kit, CpipJ_CPIJ004093 ELISA Kit

Background:

Gene Name: Coagulation Factor XI

Gene Aliases: FXI, PTA, Fletcher Factor, Prekallikrein, Plasma Thromboplastin Antecedent

Gene ID: 290757

UniProt: Q6TUF8