Complement Factor H ELISA Kit

Catalog No. ABIN5653272

Product Details Complement Factor H ELISA Kit

Target: Complement Factor H (CFH)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 12.35 ng/mL - 1000 ng/mL

Minimum Detection Limit: 12.35 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Factor H (CFH). No significant cross-reactivity or interference between Complement Factor H (CFH) and analogues was observed.

Sensitivity: 5.77 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Complement Factor H (CFH) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor H (CFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor H (CFH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor H (CFH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Alternative Name: Complement Factor H (CFH Products)

Synonyms: AHUS1 ELISA Kit, AMBP1 ELISA Kit, ARMD4 ELISA Kit, ARMS1 ELISA Kit, CFHL3 ELISA Kit, FH ELISA Kit, FHL1 ELISA Kit, HF ELISA Kit, HF1 ELISA Kit, HF2 ELISA Kit, HUS ELISA Kit, Mud-1 ELISA Kit, NOM ELISA Kit, Sas-1 ELISA Kit, Sas1 ELISA Kit, Fh ELISA Kit, CFH ELISA Kit, im:7147412 ELISA Kit, si:ch211-207o17.2 ELISA Kit, fH ELISA Kit, armd4 ELISA Kit, arms1 ELISA Kit, cfh ELISA Kit, cfhl3 ELISA Kit, fhl1 ELISA Kit, hf1 ELISA Kit, hf2 ELISA Kit, hus ELISA Kit, complement factor H ELISA Kit, complement component factor h ELISA Kit, complement factor H-related protein 5 ELISA Kit, complement factor H L homeolog ELISA Kit, CFH ELISA Kit, Cfh ELISA Kit, cfh ELISA Kit, LOC100399888 ELISA Kit, LOC100733902 ELISA Kit, cfh.L ELISA Kit

Background:

Gene Name: Complement Factor H

Gene Aliases: CF-H, FH, FHL1, ARMD4, ARMS1, CFHL3, HF1, HF2, HUS, H Factor 2, Age-Related Maculopathy Susceptibility 1, Adrenomedullin binding protein

Gene ID: 155012

UniProt: G3V9R2

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin