Corin ELISA Kit

Catalog No. ABIN5653323

Product Details Corin ELISA Kit

Target: Corin (CORIN) (Corin, Serine Peptidase (CORIN))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Corin (CRN). No significant cross-reactivity or interference between Corin (CRN) and analogues was observed.

Sensitivity: 6.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corin (CRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corin (CRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corin (CRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corin (CRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corin (CRN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corin (CRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Corin

Target: Corin (CORIN) (Corin, Serine Peptidase (CORIN))

Alternative Name: Corin (CORIN Products)

Synonyms: CORIN ELISA Kit, CG2105 ELISA Kit, Dmel\\CG2105 ELISA Kit, SP75 ELISA Kit, LOC559109 ELISA Kit, AV273130 ELISA Kit, Lrp4 ELISA Kit, ATC2 ELISA Kit, CRN ELISA Kit, PEE5 ELISA Kit, TMPRSS10 ELISA Kit, corin, serine peptidase ELISA Kit, CG2105 gene product from transcript CG2105-RC ELISA Kit, novel protein similar to H.sapiens CORIN, corin, serine peptidase (CORIN) ELISA Kit, corin ELISA Kit, CORIN ELISA Kit, Corin ELISA Kit, LOC559109 ELISA Kit

Background:

Gene Name: Corin

Gene Aliases: ATC2, CRN, Lrp4, TMPRSS10, Atrial Natriuretic Peptide-Converting Enzyme, Serine Protease, Heart-specific serine proteinase ATC2, Pro-ANP-converting enzyme

Gene ID: 53419

UniProt: Q9Z319

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones