CTH ELISA Kit

Catalog No. ABIN5653443

Product Details CTH ELISA Kit

Target: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cystathionine Gamma Lyase (CSE). No significant cross-reactivity or interference between Cystathionine Gamma Lyase (CSE) and analogues was observed.

Sensitivity: 0.121 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystathionine Gamma Lyase (CSE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystathionine Gamma Lyase (CSE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystathionine Gamma Lyase (CSE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystathionine Gamma Lyase (CSE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystathionine Gamma Lyase (CSE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystathionine Gamma Lyase (CSE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CTH

Target: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Alternative Name: Cystathionine Gamma Lyase (CTH Products)

Synonyms: DYT17 ELISA Kit, DYT18 ELISA Kit, DYT9 ELISA Kit, EIG12 ELISA Kit, GLUT ELISA Kit, GLUT1 ELISA Kit, GLUT1DS ELISA Kit, HTLVR ELISA Kit, PED ELISA Kit, wu:fb48e03 ELISA Kit, wu:fb58d08 ELISA Kit, zgc:66001 ELISA Kit, zgc:85785 ELISA Kit, CTH ELISA Kit, 0610010I13Rik ELISA Kit, AI314617 ELISA Kit, BC019483 ELISA Kit, CSE ELISA Kit, Cys3 ELISA Kit, cysA ELISA Kit, SCQ11.03c ELISA Kit, DDBDRAFT_0190313 ELISA Kit, DDBDRAFT_0191318 ELISA Kit, DDB_0190313 ELISA Kit, DDB_0191318 ELISA Kit, H ELISA Kit, MGC75946 ELISA Kit, cystathionine gamma-lyase ELISA Kit, solute carrier family 2 member 1 ELISA Kit, cystathionase (cystathionine gamma-lyase) ELISA Kit, cystathionine gamma-lyase L homeolog ELISA Kit, CTH ELISA Kit, SLC2A1 ELISA Kit, cth ELISA Kit, Cth ELISA Kit, SCO3920 ELISA Kit, mlr1566 ELISA Kit, TA_RS00415 ELISA Kit, MAP_RS15640 ELISA Kit, SPO3344 ELISA Kit, CNN01730 ELISA Kit, Mmcs_1928 ELISA Kit, RHA1_RS31820 ELISA Kit, HNE_1212 ELISA Kit, MAV_3867 ELISA Kit, MSMEG_2394 ELISA Kit, Mvan_2142 ELISA Kit, SACE_6136 ELISA Kit, Mflv_4220 ELISA Kit, cysA ELISA Kit, cth.L ELISA Kit

Background:

Gene Name: Cystathionine Gamma Lyase

Gene Aliases: CTH, Cystathionase, Cysteine-protein sulfhydrase, Gamma-cystathionase

Gene ID: 1491

UniProt: P32929

Pathways: ER-Nucleus Signaling, Warburg Effect