D Amino Acid Oxidase ELISA Kit

Catalog No. ABIN5653538

Product Details D Amino Acid Oxidase ELISA Kit

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of D-Amino Acid Oxidase (DAO). No significant cross-reactivity or interference between D-Amino Acid Oxidase (DAO) and analogues was observed.

Sensitivity: 0.56 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Amino Acid Oxidase (DAO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Amino Acid Oxidase (DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Amino Acid Oxidase (DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Amino Acid Oxidase (DAO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for D Amino Acid Oxidase

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Alternative Name: D-Amino Acid Oxidase (DAO Products)

Synonyms: DAAO ELISA Kit, DAMOX ELISA Kit, OXDA ELISA Kit, daao ELISA Kit, oxda ELISA Kit, damox ELISA Kit, DAO ELISA Kit, Dao1 ELISA Kit, DKFZp469N2232 ELISA Kit, AI987963 ELISA Kit, Dao-1 ELISA Kit, SC5F2A.23c ELISA Kit, wu:fb55b11 ELISA Kit, zgc:113922 ELISA Kit, ABP ELISA Kit, ABP1 ELISA Kit, DAO1 ELISA Kit, KAO ELISA Kit, MGC114783 ELISA Kit, Abp ELISA Kit, Abp1 ELISA Kit, D-amino acid oxidase ELISA Kit, D-amino-acid oxidase ELISA Kit, D-amino-acid oxidase, tandem duplicate 1 ELISA Kit, amine oxidase, copper containing 1 ELISA Kit, D-amino-acid oxidase L homeolog ELISA Kit, DAO ELISA Kit, Dao ELISA Kit, dao ELISA Kit, SCO6740 ELISA Kit, dao.1 ELISA Kit, AOC1 ELISA Kit, PTRG_02224 ELISA Kit, SJAG_02496 ELISA Kit, dao.L ELISA Kit, Aoc1 ELISA Kit

Background:

Gene Name: D-Amino Acid Oxidase

Gene Aliases: DAMOX