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DEFB119 ELISA Kit

DEFB119 Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5653585
  • Target See all DEFB119 ELISA Kits
    DEFB119 (Defensin, beta 119 (DEFB119))
    Reactivity
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Defensin Beta 119 (DEFb119). No significant cross-reactivity or interference between Defensin Beta 119 (DEFb119) and analogues was observed.
    Sensitivity
    0.128 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 119 (DEFb119). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Beta 119 (DEFb119). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Beta 119 (DEFb119), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Beta 119 (DEFb119) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 119 (DEFb119) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 119 (DEFb119) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all DEFB119 ELISA Kits
    DEFB119 (Defensin, beta 119 (DEFB119))
    Alternative Name
    Defensin Beta 119 (DEFB119 Products)
    Synonyms
    DEFB-19 ELISA Kit, DEFB-20 ELISA Kit, DEFB120 ELISA Kit, ESC42-RELA ELISA Kit, ESC42-RELB ELISA Kit, BBD119 ELISA Kit, BD-19 ELISA Kit, Defb24 ELISA Kit, Tdl ELISA Kit, mBD-19 ELISA Kit, CBD120 ELISA Kit, DEFB119 ELISA Kit, Defb119 ELISA Kit, defb120 ELISA Kit, defensin beta 119 ELISA Kit, defensin, beta 119 ELISA Kit, defensin beta 19 ELISA Kit, defensin beta 20 ELISA Kit, beta-defensin 119 ELISA Kit, DEFB119 ELISA Kit, Defb19 ELISA Kit, Defb20 ELISA Kit, LOC100157086 ELISA Kit, LOC100735126 ELISA Kit
    Background

    Gene Name: Defensin Beta 119

    Gene Aliases: DEFB120, DEFB-19, DEFB-20

    UniProt
    Q8N690
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