TSC22D3 ELISA Kit

Catalog No. ABIN5653614

Product Details TSC22D3 ELISA Kit

Target: TSC22D3 (TSC22 Domain Family, Member 3 (TSC22D3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 18.52 pg/mL - 1500 pg/mL

Minimum Detection Limit: 18.52 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Delta-Sleep Inducing Peptide (dSIP). No significant cross-reactivity or interference between Delta-Sleep Inducing Peptide (dSIP) and analogues was observed.

Sensitivity: 7.15 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Delta-Sleep Inducing Peptide (dSIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Delta-Sleep Inducing Peptide (dSIP) and unlabeled Delta-Sleep Inducing Peptide (dSIP) (Standards or samples) with the pre-coated antibody specific to Delta-Sleep Inducing Peptide (dSIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Delta-Sleep Inducing Peptide (dSIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Delta-Sleep Inducing Peptide (dSIP) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Delta-Sleep Inducing Peptide (dSIP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Delta-Sleep Inducing Peptide (dSIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TSC22D3

Target: TSC22D3 (TSC22 Domain Family, Member 3 (TSC22D3))

Alternative Name: Delta-Sleep Inducing Peptide (TSC22D3 Products)

Synonyms: dsipi ELISA Kit, MGC53285 ELISA Kit, zgc:65831 ELISA Kit, zgc:77028 ELISA Kit, TSC22D3 ELISA Kit, GILZ ELISA Kit, gilz ELISA Kit, tsc-22r ELISA Kit, DIP ELISA Kit, DSIPI ELISA Kit, TSC-22R ELISA Kit, hDIP ELISA Kit, Dsip1 ELISA Kit, Gilz ELISA Kit, Tilz3 ELISA Kit, Dsipi ELISA Kit, DSIP ELISA Kit, TSC22 domain family member 3 L homeolog ELISA Kit, TSC22 domain family, member 3 ELISA Kit, TSC22 domain family member 3 ELISA Kit, tsc22d3.L ELISA Kit, tsc22d3 ELISA Kit, TSC22D3 ELISA Kit, Tsc22d3 ELISA Kit

Background:

Gene Name: Delta-Sleep Inducing Peptide

Gene Aliases: DSIPI, TSC22D3, DIP, GILZ, TSC-22R, hDIP, TSC22 Domain Family Protein 3, Glucocorticoid-Induced Leucine Zipper, Delta Sleep Inducing Peptide,Immunoreactor

Gene ID: 1831

UniProt: Q99576