Desmoplakin ELISA Kit

Catalog No. ABIN5653640

Product Details Desmoplakin ELISA Kit

Target: Desmoplakin (DSP)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Desmoplakin (DSP). No significant cross-reactivity or interference between Desmoplakin (DSP) and analogues was observed.

Sensitivity: 0.113 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmoplakin (DSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Desmoplakin (DSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Desmoplakin (DSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Desmoplakin (DSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Desmoplakin (DSP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Desmoplakin (DSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: Desmoplakin (DSP Products)

Synonyms: DP ELISA Kit, DPI ELISA Kit, DPII ELISA Kit, DSP ELISA Kit, dpi ELISA Kit, dpii ELISA Kit, 2300002E22Rik ELISA Kit, 5730453H04Rik ELISA Kit, AA407887 ELISA Kit, AA407888 ELISA Kit, AW109828 ELISA Kit, im:6911953 ELISA Kit, sb:cb570 ELISA Kit, wu:fc17a08 ELISA Kit, wu:fk73d01 ELISA Kit, desmoplakin ELISA Kit, Desmoplakin ELISA Kit, ORF113 ELISA Kit, desmoplakin a ELISA Kit, ORF112 DESMOPLAKIN ELISA Kit, orf102 ELISA Kit, orf91; similar to Cydia pomonella granulovirus orf112 ELISA Kit, ORF62 ELISA Kit, ORF-66 ELISA Kit, ORF57 ELISA Kit, agip105 ELISA Kit, similar to XcORF133; similar to HaGV desmoplakin ELISA Kit, desmoplakin L homeolog ELISA Kit, ORF96 ELISA Kit, DSP ELISA Kit, PhopGV104 ELISA Kit, ORF-59 desmoplakin ELISA Kit, desmop ELISA Kit, Dsp ELISA Kit, dsp ELISA Kit, desmoplakin ELISA Kit, dspa ELISA Kit, orf112 desmoplakin ELISA Kit, ClgVgp102 ELISA Kit, COGV_gp091 ELISA Kit, APNV_p087 ELISA Kit, CBNV_gp066 ELISA Kit, EONV_gp057 ELISA Kit, OLNV_gp066 ELISA Kit, AgipMNPV_gp105 ELISA Kit, HaMNV_gp106 ELISA Kit, EupsNPV_gp065 ELISA Kit, PsunGV_gp139 ELISA Kit, dsp.L ELISA Kit, ClanGV_gp096 ELISA Kit

Background:

Gene Name: Desmoplakin

Gene Aliases: DPI, DPII, KPPS2, PPKS2, DP, 250/210 kDa paraneoplastic pemphigus antigen

Gene ID: 1832

UniProt: P15924

Pathways: Cell-Cell Junction Organization