DHODH ELISA Kit

Catalog No. ABIN5653671

This Colorimetric ELISA kit is designed for the quantitative measurement of Human DHODH.

Quick Overview for DHODH ELISA Kit (ABIN5653671)

Target: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details DHODH ELISA Kit

Minimum Detection Limit: 0.625 ng/mL

Purpose: Human Dihydroorotate Dehydrogenase (DHODH) ELISA Kit for detecting DHODH in tissue homogenates, cell lysates

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dihydroorotate Dehydrogenase (DHODH). No significant cross-reactivity or interference between Dihydroorotate Dehydrogenase (DHODH) and analogues was observed.

Sensitivity: 0.263 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydroorotate Dehydrogenase (DHODH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydroorotate Dehydrogenase (DHODH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydroorotate Dehydrogenase (DHODH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydroorotate Dehydrogenase (DHODH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydroorotate Dehydrogenase (DHODH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydroorotate Dehydrogenase (DHODH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DHODH

Target: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Alternative Name: Dihydroorotate Dehydrogenase

Background: Gene Aliases: DHOdehase, Dihydroorotate oxidase, Dihydroorotate dehydrogenase (quinone) , mitochondrial

Gene ID: 1723

UniProt: Q02127

Pathways: Ribonucleoside Biosynthetic Process, Protein targeting to Nucleus