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DPP9 ELISA Kit

DPP9 Reactivity: Mouse Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Tissue Homogenate
Catalog No. ABIN5653689
  • Target See all DPP9 ELISA Kits
    DPP9 (Dipeptidyl-Peptidase 9 (DPP9))
    Reactivity
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.12 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78.12 pg/mL
    Application
    ELISA
    Sample Type
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase 9 (DPP9). No significant cross-reactivity or interference between Dipeptidyl Peptidase 9 (DPP9) and analogues was observed.
    Sensitivity
    25 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 9 (DPP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 9 (DPP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 9 (DPP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase 9 (DPP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidyl Peptidase 9 (DPP9) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidyl Peptidase 9 (DPP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all DPP9 ELISA Kits
    DPP9 (Dipeptidyl-Peptidase 9 (DPP9))
    Alternative Name
    Dipeptidyl Peptidase 9 (DPP9 Products)
    Synonyms
    DP9 ELISA Kit, DPLP9 ELISA Kit, DPRP-2 ELISA Kit, DPRP2 ELISA Kit, 6430584G11Rik ELISA Kit, A330078I11 ELISA Kit, DPP IX ELISA Kit, im:7145318 ELISA Kit, zgc:152900 ELISA Kit, dpp9 ELISA Kit, dipeptidyl peptidase 9 ELISA Kit, dipeptidylpeptidase 9 ELISA Kit, dipeptidyl-peptidase 9 ELISA Kit, dipeptidyl-peptidase 9 L homeolog ELISA Kit, DPP9 ELISA Kit, Dpp9 ELISA Kit, dpp9 ELISA Kit, dpp9.L ELISA Kit
    Background

    Gene Name: Dipeptidyl Peptidase 9

    Gene Aliases: DPRP2, DPLP9, Dipeptidylpeptidase 9, Dipeptidyl peptidase IV-related protein 2, Dipeptidyl peptidase-like protein 9, Dipeptidyl peptidase IX

    Gene ID
    224897
    UniProt
    Q8BVG4
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