Docosahexaenoic Acid ELISA Kit
Quick Overview for Docosahexaenoic Acid ELISA Kit (ABIN5653715)
Target
Reactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 12.35 pg/mL
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Analytical Method
- Quantitative
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Specificity
- This assay has high sensitivity and excellent specificity for detection of Docosahexaenoic Acid (DHA). No significant cross-reactivity or interference between Docosahexaenoic Acid (DHA) and analogues was observed.
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Sensitivity
- 4.93 pg/mL
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Docosahexaenoic Acid ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
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Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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Assay Time
- 2 h
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Plate
- Pre-coated
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Protocol
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Docosahexaenoic Acid (DHA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Docosahexaenoic Acid (DHA) and unlabeled Docosahexaenoic Acid (DHA) (Standards or samples) with the pre-coated antibody specific to Docosahexaenoic Acid (DHA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Docosahexaenoic Acid (DHA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Docosahexaenoic Acid (DHA) in the sample.
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Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Docosahexaenoic Acid (DHA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Docosahexaenoic Acid (DHA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
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Storage
- 4 °C,-20 °C
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Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
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Expiry Date
- 4-8 months
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- Docosahexaenoic Acid
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Target Type
- Chemical
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Background
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Gene Name: Docosahexaenoic Acid
Gene Aliases: Doconexent, Cervonic Acid
Target
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