DDC ELISA Kit

Catalog No. ABIN5653718

Product Details DDC ELISA Kit

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Dopa Decarboxylase (DDC). No significant cross-reactivity or interference between Dopa Decarboxylase (DDC) and analogues was observed.

Sensitivity: 0.61 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopa Decarboxylase (DDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopa Decarboxylase (DDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopa Decarboxylase (DDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopa Decarboxylase (DDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopa Decarboxylase (DDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopa Decarboxylase (DDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DDC

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Alternative Name: Dopa Decarboxylase (DDC Products)

Synonyms: AADC ELISA Kit, wu:fa56d05 ELISA Kit, wu:fd59h03 ELISA Kit, wu:fk20h01 ELISA Kit, zgc:65801 ELISA Kit, zgc:76929 ELISA Kit, Ddc ELISA Kit, DdcDv ELISA Kit, Dvir\\GJ17995 ELISA Kit, GJ17995 ELISA Kit, dvir_GLEANR_2561 ELISA Kit, Aadc ELISA Kit, 5-HT ELISA Kit, CG10697 ELISA Kit, DDC ELISA Kit, DdcDm ELISA Kit, Dmel\\CG10697 ELISA Kit, ddc ELISA Kit, fDDC ELISA Kit, l(2)37Bl ELISA Kit, l(2)37Ch ELISA Kit, l(2)k02104 ELISA Kit, dopa decarboxylase ELISA Kit, Dopa-decarboxylase ELISA Kit, dopa decarboxylase (aromatic L-amino acid decarboxylase) ELISA Kit, Dopa decarboxylase ELISA Kit, dopa decarboxylase L homeolog ELISA Kit, DDC ELISA Kit, ddc ELISA Kit, Ddc ELISA Kit, Dvir\Ddc ELISA Kit, ddc.L ELISA Kit

Background:

Gene Name: Dopa Decarboxylase

Gene Aliases: AADC, Aromatic L-Amino Acid Decarboxylase, DOPA decarboxylase

Gene ID: 1644

UniProt: P20711

Pathways: Dopaminergic Neurogenesis