Elastase 3B ELISA Kit

Catalog No. ABIN5653800

Product Details Elastase 3B ELISA Kit

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Elastase 3B (ELA3B). No significant cross-reactivity or interference between Elastase 3B (ELA3B) and analogues was observed.

Sensitivity: 0.135 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3B (ELA3B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 3B (ELA3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3B (ELA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 3B (ELA3B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Elastase 3B (ELA3B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: Elastase 3B (ELA3B Products)

Synonyms: CBPP ELISA Kit, E1 ELISA Kit, EL-1 ELISA Kit, ELA3B ELISA Kit, 0910001F22Rik ELISA Kit, 2310074F01Rik ELISA Kit, AI504000 ELISA Kit, Ela3 ELISA Kit, Ela3b ELISA Kit, ela3b ELISA Kit, MGC82867 ELISA Kit, ELA1 ELISA Kit, Cela3b ELISA Kit, CELA3B ELISA Kit, chymotrypsin like elastase family member 3B ELISA Kit, chymotrypsin-like elastase family, member 3B ELISA Kit, elastase 3B, pancreatic ELISA Kit, proproteinase E ELISA Kit, chymotrypsin like elastase family member 3B L homeolog ELISA Kit, chymotrypsin-like elastase family member 3B ELISA Kit, CELA3B ELISA Kit, Cela3b ELISA Kit, ela3b ELISA Kit, LOC478196 ELISA Kit, cela3b.L ELISA Kit, LOC100717936 ELISA Kit, LOC100071692 ELISA Kit

Background:

Gene Name: Elastase 3B

Gene Aliases: CELA3B, Chymotrypsin Like Elastase Family Member 3B, Protease E, Fecal Elastase, Elastase 1, Cholesterol-Binding Pancreatic Protease, Pancreatic Endopeptidase E

Gene ID: 23436

UniProt: P08861