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Endonuclease G ELISA Kit

ENDOG Reactivity: Rat Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5653840
  • Target See all Endonuclease G (ENDOG) ELISA Kits
    Endonuclease G (ENDOG)
    Reactivity
    • 3
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.12 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78.12 pg/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Endonuclease G, Mitochondrial (ENDOG). No significant cross-reactivity or interference between Endonuclease G, Mitochondrial (ENDOG) and analogues was observed.
    Sensitivity
    32 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endonuclease G, Mitochondrial (ENDOG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endonuclease G, Mitochondrial (ENDOG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endonuclease G, Mitochondrial (ENDOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Endonuclease G, Mitochondrial (ENDOG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endonuclease G, Mitochondrial (ENDOG) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endonuclease G, Mitochondrial (ENDOG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Endonuclease G (ENDOG) ELISA Kits
    Endonuclease G (ENDOG)
    Alternative Name
    Endonuclease G, Mitochondrial (ENDOG Products)
    Synonyms
    CG8862 ELISA Kit, Dmel\\CG8862 ELISA Kit, Tb08.10J17.140 ELISA Kit, Tb08.10J17.90 ELISA Kit, TBC1D13 ELISA Kit, endog ELISA Kit, ENDOG ELISA Kit, wu:fb79c08 ELISA Kit, zgc:110020 ELISA Kit, Endonuclease G ELISA Kit, endonuclease G ELISA Kit, putative endonuclease G ELISA Kit, endonuclease G, mitochondrial ELISA Kit, endonuclease G L homeolog ELISA Kit, EndoG ELISA Kit, Tc00.1047053506867.10 ELISA Kit, Tb927.8.4040 ELISA Kit, Tb927.8.4090 ELISA Kit, LBRM_10_0750 ELISA Kit, LMJF_10_0610 ELISA Kit, NAEGRDRAFT_78507 ELISA Kit, ENDOG ELISA Kit, endog ELISA Kit, eNDOG ELISA Kit, LOC100529218 ELISA Kit, Endog ELISA Kit, endog.L ELISA Kit
    Background

    Gene Name: Endonuclease G, Mitochondrial

    Gene Aliases: ENDO-G

    Gene ID
    362100
    UniProt
    Q3V5X8
    Pathways
    Apoptosis
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