Ecotin ELISA Kit

Catalog No. ABIN5653981

Product Details

Target: Ecotin

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Biological Agents

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Escherichia coli Protein (ECP). No significant cross-reactivity or interference between Escherichia coli Protein (ECP) and analogues was observed.

Sensitivity: 0.134 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Escherichia coli Protein (ECP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Escherichia coli Protein (ECP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Escherichia coli Protein (ECP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Escherichia coli Protein (ECP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Escherichia coli Protein (ECP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Escherichia coli Protein (ECP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Ecotin

Alternative Name: Escherichia coli Protein

Synonyms: 2 ELISA Kit, EAR-13 ELISA Kit, EAR-2 ELISA Kit, ECP ELISA Kit, ECP 2 ELISA Kit, Rnase2 ELISA Kit, EAR ELISA Kit, R1 ELISA Kit, R7 ELISA Kit, Rnase3 ELISA Kit, RNS3 ELISA Kit, eosinophil-associated, ribonuclease A family, member 2 ELISA Kit, eosinophil-associated, ribonuclease A family, member 11 ELISA Kit, ribonuclease A family member 3 ELISA Kit, Ear2 ELISA Kit, Ear11 ELISA Kit, RNASE3 ELISA Kit

Background:

Gene Name: Escherichia coli Protein

Gene ID: 946700

UniProt: P23827