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FCGBP ELISA Kit (Fc Fragment of IgG Binding Protein) ELISA Kit

FCGBP Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5654103
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96 tests
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  • Target
    Detection Method
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    This assay has high sensitivity and excellent specificity for detection of Fc Fragment Of IgG Binding Protein (FcgBP). No significant cross-reactivity or interference between Fc Fragment Of IgG Binding Protein (FcgBP) and analogues was observed.
    0.117 ng/mL
    Discover our best selling FCGBP ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fc Fragment Of IgG Binding Protein (FcgBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fc Fragment Of IgG Binding Protein (FcgBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fc Fragment Of IgG Binding Protein (FcgBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fc Fragment Of IgG Binding Protein (FcgBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fc Fragment Of IgG Binding Protein (FcgBP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fc Fragment Of IgG Binding Protein (FcgBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target
    Alternative Name
    Fc Fragment Of IgG Binding Protein (FCGBP ELISA Kit Abstract)
    FC(GAMMA)BP, A430096B05Rik, Fc fragment of IgG binding protein, FCGBP, Fcgbp

    Gene Name: Fc Fragment Of IgG Binding Protein

    Gene Aliases: FC(GAMMA)BP, IgG Fc Binding Protein, Human Fc Gamma BP, Fcgamma-binding protein antigen

    Gene ID
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