FTH1 ELISA Kit

Catalog No. ABIN5654119

Product Details FTH1 ELISA Kit

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ferritin, Heavy Polypeptide (FTH). No significant cross-reactivity or interference between Ferritin, Heavy Polypeptide (FTH) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin, Heavy Polypeptide (FTH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferritin, Heavy Polypeptide (FTH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin, Heavy Polypeptide (FTH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferritin, Heavy Polypeptide (FTH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Alternative Name: Ferritin, Heavy Polypeptide (FTH1 Products)

Synonyms: FHC ELISA Kit, FTH ELISA Kit, FTHL6 ELISA Kit, PIG15 ELISA Kit, PLIF ELISA Kit, apoferritin ELISA Kit, ferritin ELISA Kit, fhc ELISA Kit, fth ELISA Kit, fth1 ELISA Kit, fthl6 ELISA Kit, ftn-2 ELISA Kit, pig15 ELISA Kit, plif ELISA Kit, fth1b ELISA Kit, Fth ELISA Kit, HFt ELISA Kit, MFH ELISA Kit, fb06g09 ELISA Kit, hm:zeh1145 ELISA Kit, wu:fb06g09 ELISA Kit, wu:fq18c10 ELISA Kit, zeh1145 ELISA Kit, ferritin heavy chain 1 ELISA Kit, ferritin, heavy polypeptide 1 L homeolog ELISA Kit, ferritin, heavy polypeptide 1 S homeolog ELISA Kit, ferritin heavy polypeptide 1 ELISA Kit, ferritin, heavy polypeptide 1a ELISA Kit, ferritin ELISA Kit, ferritin, heavy polypeptide 1 ELISA Kit, tudor domain containing 9 ELISA Kit, ferritin, heavy polypeptide 1 a ELISA Kit, FTH1 ELISA Kit, fth1.L ELISA Kit, fth1.S ELISA Kit, Fth1 ELISA Kit, fth1a ELISA Kit, EAMY_RS19690 ELISA Kit, fth1 ELISA Kit, TDRD9 ELISA Kit

Background:

Gene Name: Ferritin, Heavy Polypeptide

Gene Aliases: FTH1, FTHL6, PIG15, PLIF, Ferritin Heavy Chain, Apoferritin, Placenta Immunoregulatory Factor, Proliferation-Inducing Protein 15, Cell proliferation-inducing gene 15 protein

Gene ID: 14319

UniProt: P09528

Pathways: Transition Metal Ion Homeostasis