FTL ELISA Kit

Catalog No. ABIN5654121

Product Details FTL ELISA Kit

Target: FTL (Ferritin, Light Polypeptide (FTL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ferritin, Light Polypeptide (FTL). No significant cross-reactivity or interference between Ferritin, Light Polypeptide (FTL) and analogues was observed.

Sensitivity: 6.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin, Light Polypeptide (FTL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferritin, Light Polypeptide (FTL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin, Light Polypeptide (FTL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferritin, Light Polypeptide (FTL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin, Light Polypeptide (FTL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin, Light Polypeptide (FTL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: Ferritin, Light Polypeptide (FTL Products)

Synonyms: NBIA3 ELISA Kit, apoferritin ELISA Kit, ferritin ELISA Kit, fhc ELISA Kit, fth ELISA Kit, fth1 ELISA Kit, fthl6 ELISA Kit, ftn-2 ELISA Kit, pig15 ELISA Kit, plif ELISA Kit, FT ELISA Kit, Ftl ELISA Kit, Ftl-1 ELISA Kit, L-ferritin ELISA Kit, FTL ELISA Kit, Ftl1 ELISA Kit, FTL2 ELISA Kit, FTD ELISA Kit, ferritin light chain ELISA Kit, ferritin, heavy polypeptide 1 L homeolog ELISA Kit, ferritin light polypeptide 1 ELISA Kit, ferritin, light polypeptide ELISA Kit, ferritin light-chain ELISA Kit, ferritin light chain 1 ELISA Kit, FTL ELISA Kit, fth1.L ELISA Kit, Ftl1 ELISA Kit, CpipJ_CPIJ004069 ELISA Kit, Ftl ELISA Kit

Background:

Gene Name: Ferritin, Light Polypeptide

Gene Aliases: Ferritin Light Chain, Ferritin Light Polypeptide-Like 3, L Apoferritin, Ferritin L Subunit

Gene ID: 2512

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis