FGF12 CLIA Kit

Catalog No. ABIN5654201

Mouse FGF12 ELISA Kit, Chemiluminescent assay for quantification of Mouse FGF12.

Quick Overview for FGF12 CLIA Kit (ABIN5654201)

Target: FGF12 (Fibroblast Growth Factor 12 (FGF12))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 1.37 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details FGF12 CLIA Kit

Minimum Detection Limit: 1.37 pg/mL

Purpose: Mouse Fibroblast Growth Factor 12 (FGF12) CLIA Kit for detecting FGF12 in serum, plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 12 (FGF12). No significant cross-reactivity or interference between Fibroblast Growth Factor 12 (FGF12) and analogues was observed.

Sensitivity: 0.61 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 12 (FGF12). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 12 (FGF12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibroblast Growth Factor 12 (FGF12) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 12 (FGF12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 12 (FGF12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: FGF12 (Fibroblast Growth Factor 12 (FGF12))

Alternative Name: Fibroblast Growth Factor 12

Background: Gene Aliases: FGF12B, FHF1, Fibroblast Growth Factor Homologous Factor 1, Myocyte-Activating Factor

Gene ID: 14167

UniProt: P61329

Pathways: Negative Regulation of Transporter Activity