FGF15 ELISA Kit

Catalog No. ABIN5654216

Mouse FGF15 ELISA Kit, Colorimetric assay for quantification of Mouse FGF15.

Quick Overview for FGF15 ELISA Kit (ABIN5654216)

Target: FGF15 (Fibroblast Growth Factor 15 (FGF15))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 6.25 pg/mL - 100 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Product Details FGF15 ELISA Kit

Minimum Detection Limit: 6.25 pg/mL

Purpose: Mouse Fibroblast Growth Factor 15 (FGF15) High-sensitive ELISA Kit for detecting FGF15 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 15 (FGF15). No significant cross-reactivity or interference between Fibroblast Growth Factor 15 (FGF15) and analogues was observed.

Sensitivity: 2.62 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 50 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 15 (FGF15) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 15 (FGF15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FGF15

Target: FGF15 (Fibroblast Growth Factor 15 (FGF15))

Alternative Name: Fibroblast Growth Factor 15

Background: Gene Aliases:

Gene ID: 14170

UniProt: O35622