FOXP1 ELISA Kit

Catalog No. ABIN5654412

Product Details FOXP1 ELISA Kit

Target: FOXP1 (Forkhead Box P1 (FOXP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Forkhead Box Protein P1 (FOXP1). No significant cross-reactivity or interference between Forkhead Box Protein P1 (FOXP1) and analogues was observed.

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein P1 (FOXP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein P1 (FOXP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein P1 (FOXP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein P1 (FOXP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein P1 (FOXP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein P1 (FOXP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FOXP1

Target: FOXP1 (Forkhead Box P1 (FOXP1))

Alternative Name: Forkhead Box Protein P1 (FOXP1 Products)

Synonyms: 12CC4 ELISA Kit, QRF1 ELISA Kit, hFKH1B ELISA Kit, 3110052D19Rik ELISA Kit, 4932443N09Rik ELISA Kit, AI461938 ELISA Kit, AW494214 ELISA Kit, foxp1 ELISA Kit, im:7139058 ELISA Kit, wu:fc83a06 ELISA Kit, FOXP1 ELISA Kit, MGC145686 ELISA Kit, 12cc4 ELISA Kit, fkh1b ELISA Kit, hspc215 ELISA Kit, qrf1 ELISA Kit, xlfoxp1 ELISA Kit, im:7146611 ELISA Kit, wu:fb97f01 ELISA Kit, zgc:154010 ELISA Kit, forkhead box P1 ELISA Kit, forkhead box P1b ELISA Kit, forkhead box P1 S homeolog ELISA Kit, forkhead box P1a ELISA Kit, FOXP1 ELISA Kit, Foxp1 ELISA Kit, foxp1b ELISA Kit, foxp1 ELISA Kit, foxp1.S ELISA Kit, foxp1a ELISA Kit

Gene ID: 108655

UniProt: P58462

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response