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GALT ELISA Kit

GALT Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5654467
  • Target See all GALT ELISA Kits
    GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
    Reactivity
    • 3
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Galactose-1-Phosphate Uridylyltransferase (GALT). No significant cross-reactivity or interference between Galactose-1-Phosphate Uridylyltransferase (GALT) and analogues was observed.
    Sensitivity
    0.056 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactose-1-Phosphate Uridylyltransferase (GALT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactose-1-Phosphate Uridylyltransferase (GALT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactose-1-Phosphate Uridylyltransferase (GALT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galactose-1-Phosphate Uridylyltransferase (GALT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galactose-1-Phosphate Uridylyltransferase (GALT) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galactose-1-Phosphate Uridylyltransferase (GALT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all GALT ELISA Kits
    GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))
    Alternative Name
    Galactose-1-Phosphate Uridylyltransferase (GALT Products)
    Synonyms
    GALT ELISA Kit, AW553376 ELISA Kit, CG9232 ELISA Kit, Dmel\\CG9232 ELISA Kit, dGALT ELISA Kit, galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridylyltransferase, GalT ELISA Kit, probable galactose-1-phosphate uridylyltransferase galtb [second part] ELISA Kit, UDP-glucose--galactose-1-phosphate uridylyltransferase ELISA Kit, GalT galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridyl transferase ELISA Kit, Galactose-1-phosphate uridylyltransferase ELISA Kit, galactose-1-phosphate uridylyltransferase S homeolog ELISA Kit, GALT ELISA Kit, galT ELISA Kit, Galt ELISA Kit, CNM00620 ELISA Kit, Mrub_2813 ELISA Kit, Mesil_2395 ELISA Kit, Trad_0989 ELISA Kit, Igag_1846 ELISA Kit, VDIS_RS10425 ELISA Kit, Intca_2810 ELISA Kit, Marky_2157 ELISA Kit, Selsp_1876 ELISA Kit, Trebr_2368 ELISA Kit, Halhy_5538 ELISA Kit, Theth_1357 ELISA Kit, galt.S ELISA Kit
    Background

    Gene Name: Galactose-1-Phosphate Uridylyltransferase

    Gene Aliases: UDP-glucose--hexose-1-phosphate uridylyltransferase

    Gene ID
    298003
    UniProt
    P43424
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