GLB1 ELISA Kit

Catalog No. ABIN5654470

Product Details GLB1 ELISA Kit

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Minimum Detection Limit: 125 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Galactosidase Beta (GLb). No significant cross-reactivity or interference between Galactosidase Beta (GLb) and analogues was observed.

Sensitivity: 54 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactosidase Beta (GLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactosidase Beta (GLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactosidase Beta (GLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galactosidase Beta (GLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galactosidase Beta (GLb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galactosidase Beta (GLb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: Galactosidase Beta (GLB1 Products)

Synonyms: EBP ELISA Kit, ELNR1 ELISA Kit, MPS4B ELISA Kit, DER ELISA Kit, XR ELISA Kit, 0610038K04Rik ELISA Kit, 1810027P18Rik ELISA Kit, glb ELISA Kit, CG9092 ELISA Kit, Dmel\\CG9092 ELISA Kit, beta-GAL ELISA Kit, beta-Gal-1 ELISA Kit, beta-gal ELISA Kit, gal ELISA Kit, lacZ-1 ELISA Kit, AW125515 ELISA Kit, Bge ELISA Kit, Bgl ELISA Kit, Bgl-e ELISA Kit, Bgl-s ELISA Kit, Bgl-t ELISA Kit, Bgs ELISA Kit, Bgt ELISA Kit, C130097A14Rik ELISA Kit, BGAL ELISA Kit, zgc:110823 ELISA Kit, ECK0341 ELISA Kit, JW0335 ELISA Kit, GLB ELISA Kit, galactosidase beta 1 ELISA Kit, dicarbonyl and L-xylulose reductase ELISA Kit, dicarbonyl L-xylulose reductase ELISA Kit, beta-D-galactosidase ELISA Kit, beta-galactosidase ELISA Kit, beta galactosidase ELISA Kit, galactosidase, beta 1 ELISA Kit, beta-galactosidase-like ELISA Kit, GLB1 ELISA Kit, DCXR ELISA Kit, Dcxr ELISA Kit, lacZ ELISA Kit, SSP_RS00525 ELISA Kit, lacH ELISA Kit, ECL_03691 ELISA Kit, Gal ELISA Kit, Glb1 ELISA Kit, glb1 ELISA Kit, VV2_1327 ELISA Kit, LOC100056371 ELISA Kit

Background:

Gene Name: Galactosidase Beta

Gene Aliases: GL-B, b-GAL, bGAL, ELNR1, EBP, GLB1, Lactase, Elastin Receptor 1,67 kDa, Elastin Receptor 1(67kD), Acid Beta-Galactosidase

Gene ID: 2720

UniProt: P16278

Pathways: Glycosaminoglycan Metabolic Process