Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Galectin 9 ELISA Kit

LGALS9 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5654494
  • Target See all Galectin 9 (LGALS9) ELISA Kits
    Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))
    Reactivity
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    7.8 pg/mL - 500 pg/mL
    Minimum Detection Limit
    7.8 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Galectin 9 (GAL9). No significant cross-reactivity or interference between Galectin 9 (GAL9) and analogues was observed.
    Sensitivity
    2.9 pg/mL
    Featured
    Discover our best selling LGALS9 ELISA Kit
    Top Product
    Discover our top product LGALS9 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 9 (GAL9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 9 (GAL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 9 (GAL9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galectin 9 (GAL9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 9 (GAL9) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 9 (GAL9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Galectin 9 (LGALS9) ELISA Kits
    Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))
    Alternative Name
    Galectin 9 (LGALS9 Products)
    Synonyms
    huat ELISA Kit, lgals9a ELISA Kit, ecalectin ELISA Kit, galectin-9 ELISA Kit, Lgals9 ELISA Kit, wu:fd20c09 ELISA Kit, zgc:111833 ELISA Kit, LGALS9 ELISA Kit, AA407335 ELISA Kit, AI194909 ELISA Kit, AI265545 ELISA Kit, LGALS35 ELISA Kit, Lgals5 ELISA Kit, gal-9 ELISA Kit, HUAT ELISA Kit, LGALS9A ELISA Kit, UAT ELISA Kit, UATP.I ELISA Kit, galectin 9 L homeolog ELISA Kit, galectin 9 ELISA Kit, lectin, galactoside-binding, soluble, 9 (galectin 9)-like 3 ELISA Kit, lectin, galactose binding, soluble 9 ELISA Kit, lgals9.L ELISA Kit, lgals9 ELISA Kit, lgals9l3 ELISA Kit, LGALS9 ELISA Kit, Lgals9 ELISA Kit
    Background

    Gene Name: Galectin 9

    Gene Aliases: LGALS9, Ecalectin, Lectin,Galactoside-Binding Soluble 9, Tumor antigen HOM-HD-21

    Gene ID
    16859
    UniProt
    O08573
You are here:
Support