Glucose-6-Phosphate Dehydrogenase ELISA Kit

Catalog No. ABIN5654612

Product Details Glucose-6-Phosphate Dehydrogenase ELISA Kit

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glucose 6 Phosphate Dehydrogenase (G6PD). No significant cross-reactivity or interference between Glucose 6 Phosphate Dehydrogenase (G6PD) and analogues was observed.

Sensitivity: 0.138 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Dehydrogenase (G6PD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Dehydrogenase (G6PD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Dehydrogenase (G6PD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose 6 Phosphate Dehydrogenase (G6PD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose 6 Phosphate Dehydrogenase (G6PD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: Glucose 6 Phosphate Dehydrogenase (G6PD Products)

Synonyms: G6PD1 ELISA Kit, g6pd ELISA Kit, G6pd ELISA Kit, G6pdx ELISA Kit, g6pdh ELISA Kit, g6pd2 ELISA Kit, fj78b06 ELISA Kit, wu:fj78b06 ELISA Kit, si:dkey-90a13.8 ELISA Kit, BA3433 ELISA Kit, CORTRD1 ELISA Kit, G6PDH ELISA Kit, GDH ELISA Kit, G28A ELISA Kit, G6PD ELISA Kit, Gpdx ELISA Kit, glucose-6-phosphate dehydrogenase ELISA Kit, glucose-6-phosphate 1-dehydrogenase ELISA Kit, glucose-6-phosphate 1-dehydrogenase (G6PD) ELISA Kit, glucose-6-P dehydrogenase ELISA Kit, glucose-6-phosphate dehydrogenase L homeolog ELISA Kit, glucose-6-phosphate 1-dehydrogenase Zwf ELISA Kit, hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase ELISA Kit, glucose-6-phosphate dehydrogenase X-linked ELISA Kit, G6PD ELISA Kit, g6pd ELISA Kit, g6pD ELISA Kit, zwf ELISA Kit, LOC100120232 ELISA Kit, LACBIDRAFT_188936 ELISA Kit, G6pd ELISA Kit, g6pd.L ELISA Kit, CNG03280 ELISA Kit, Tb10.70.5200 ELISA Kit, H6PD ELISA Kit, G6pdx ELISA Kit, H6pd ELISA Kit

Background:

Gene Name: Glucose 6 Phosphate Dehydrogenase

Gene Aliases: Glucose-6-phosphate 1-dehydrogenase

Gene ID: 14381

UniProt: Q00612

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones