GPI ELISA Kit

Catalog No. ABIN5654614

Product Details GPI ELISA Kit

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glucose 6 Phosphate Isomerase (GPI). No significant cross-reactivity or interference between Glucose 6 Phosphate Isomerase (GPI) and analogues was observed.

Sensitivity: 0.063 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Isomerase (GPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Isomerase (GPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Isomerase (GPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Isomerase (GPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose 6 Phosphate Isomerase (GPI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose 6 Phosphate Isomerase (GPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Alternative Name: Glucose 6 Phosphate Isomerase (GPI Products)

Synonyms: PGI ELISA Kit, Amf ELISA Kit, Gpi1 ELISA Kit, GPI ELISA Kit, DDBDRAFT_0185620 ELISA Kit, DDBDRAFT_0231026 ELISA Kit, DDB_0185620 ELISA Kit, DDB_0231026 ELISA Kit, CG8251 ELISA Kit, Dmel\\CG8251 ELISA Kit, Gpi ELISA Kit, pgi ELISA Kit, Gpi-1 ELISA Kit, Gpi-1r ELISA Kit, Gpi-1s ELISA Kit, Gpi-1t ELISA Kit, Gpi1-r ELISA Kit, Gpi1-s ELISA Kit, Gpi1-t ELISA Kit, Gpi1s ELISA Kit, MF ELISA Kit, NK ELISA Kit, NK/GPI ELISA Kit, Org ELISA Kit, AMF ELISA Kit, GNPI ELISA Kit, NLK ELISA Kit, PHI ELISA Kit, SA-36 ELISA Kit, SA36 ELISA Kit, gpi ELISA Kit, pgi-2 ELISA Kit, glucose-6-phosphate isomerase ELISA Kit, gpi ELISA Kit, glucose-6-phosphate isomerase 1 ELISA Kit, gpi Glucose-6-phosphate isomerase ELISA Kit, Phosphoglucose isomerase ELISA Kit, glucose phosphate isomerase 1 ELISA Kit, glucose-6-phosphate isomerase b ELISA Kit, GPI ELISA Kit, Gpi ELISA Kit, gpi ELISA Kit, pgi ELISA Kit, GPI1 ELISA Kit, Pgi ELISA Kit, Gpi1 ELISA Kit, gpib ELISA Kit

Target Type: Viral Protein

Background:

Gene Name: Glucose 6 Phosphate Isomerase

Gene Aliases: AMF, NLK, PHI, SA36, Phosphoglucose Isomerase, Autocrine motility factor, Neuroleukin, Phosphohexose isomerase, Sperm antigen 36

Gene ID: 2821

UniProt: P06744