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GCLC ELISA Kit

GCLC Reactivity: Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5654642
  • Target See all GCLC ELISA Kits
    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
    Reactivity
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Glutamate Cysteine Ligase, Catalytic (GCLC). No significant cross-reactivity or interference between Glutamate Cysteine Ligase, Catalytic (GCLC) and analogues was observed.
    Sensitivity
    0.126 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, Catalytic (GCLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, Catalytic (GCLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Cysteine Ligase, Catalytic (GCLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Cysteine Ligase, Catalytic (GCLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Catalytic (GCLC) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Catalytic (GCLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all GCLC ELISA Kits
    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
    Alternative Name
    Glutamate Cysteine Ligase, Catalytic (GCLC Products)
    Synonyms
    GCLC ELISA Kit, gclc ELISA Kit, MGC146287 ELISA Kit, 2259 ELISA Kit, CG2259 ELISA Kit, DmGCLC ELISA Kit, DmGCS ELISA Kit, DmGCSh ELISA Kit, Dmel\\CG2259 ELISA Kit, GCL ELISA Kit, GCLc ELISA Kit, GCSh ELISA Kit, Gcs ELISA Kit, Gcsh ELISA Kit, DDBDRAFT_0186120 ELISA Kit, DDBDRAFT_0231403 ELISA Kit, DDB_0186120 ELISA Kit, DDB_0231403 ELISA Kit, GCS ELISA Kit, GLCL ELISA Kit, GLCLC ELISA Kit, D9Wsu168e ELISA Kit, GLCL-H ELISA Kit, Ggcs-hs ELISA Kit, Glclc ELISA Kit, cb1049 ELISA Kit, fe36e11 ELISA Kit, wu:fe36e11 ELISA Kit, glutamate-cysteine ligase catalytic subunit ELISA Kit, glutamate-cysteine ligase, catalytic subunit ELISA Kit, Glutamate-cysteine ligase catalytic subunit ELISA Kit, gamma glutamylcysteine synthetase ELISA Kit, glutamate-cysteine ligase, catalytic subunit L homeolog ELISA Kit, glutamate-cysteine ligase, catalytic subunit S homeolog ELISA Kit, gamma-glutamylcysteine synthetase ELISA Kit, GCLC ELISA Kit, gclc ELISA Kit, Gclc ELISA Kit, LbGCS ELISA Kit, gcsA ELISA Kit, gclc.L ELISA Kit, gclc.S ELISA Kit, GSH1 ELISA Kit
    Background

    Gene Name: Glutamate Cysteine Ligase, Catalytic

    Gene Aliases: GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS

    Gene ID
    25283
    UniProt
    P19468
    Pathways
    Cell RedoxHomeostasis
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