GAD65 ELISA Kit

Catalog No. ABIN5654650

The Human GAD65 ELISA Kit (ABIN5654650) is a Colorimetric ELISA Kit designed to quantify Human GAD65.

Quick Overview for GAD65 ELISA Kit (ABIN5654650)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Product Details GAD65 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Purpose: Human Glutamate Decarboxylase 2 (GAD2) ELISA Kit for detecting GAD2 in tissue homogenates, cell lysates, cell culture supernates

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Decarboxylase 2, Acid (GAD2). No significant cross-reactivity or interference between Glutamate Decarboxylase 2, Acid (GAD2) and analogues was observed.

Sensitivity: 0.27 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Decarboxylase 2, Acid (GAD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Decarboxylase 2, Acid (GAD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Decarboxylase 2, Acid (GAD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Decarboxylase 2, Acid (GAD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Decarboxylase 2, Acid (GAD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Decarboxylase 2, Acid (GAD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 12 months

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: Glutamate Decarboxylase 2

Background: Gene Aliases: GAD65, Glutamate Decarboxylase 2, Acid, Pancreatic Islets And Brain,65 kDa, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform

Gene ID: 2572

UniProt: Q05329