GLUD1 ELISA Kit

Catalog No. ABIN5654655

Product Details GLUD1 ELISA Kit

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Dehydrogenase 1 (GDH). No significant cross-reactivity or interference between Glutamate Dehydrogenase 1 (GDH) and analogues was observed.

Sensitivity: 0.138 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Dehydrogenase 1 (GDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Dehydrogenase 1 (GDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Dehydrogenase 1 (GDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Dehydrogenase 1 (GDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Dehydrogenase 1 (GDH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Dehydrogenase 1 (GDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: Glutamate Dehydrogenase 1 (GLUD1 Products)

Synonyms: GDH ELISA Kit, gdh1 ELISA Kit, GDH 1 ELISA Kit, glud1 ELISA Kit, GLUD1 ELISA Kit, cb719 ELISA Kit, wu:fb16e02 ELISA Kit, wu:fb58f12 ELISA Kit, wu:fe37f03 ELISA Kit, wu:fj43f02 ELISA Kit, zgc:192851 ELISA Kit, zgc:55630 ELISA Kit, GDH1 ELISA Kit, GLUD ELISA Kit, AI118167 ELISA Kit, Gdh-X ELISA Kit, Glud ELISA Kit, Gludl ELISA Kit, Ac2-281 ELISA Kit, Gdh1 ELISA Kit, Gludeha ELISA Kit, MRG-2 ELISA Kit, GLUTAMATE DECARBOXYLASE 1 ELISA Kit, GLUTAMATE DEHYDROGENASE 1 ELISA Kit, MRG7.13 ELISA Kit, MRG7_13 ELISA Kit, glutamate dehydrogenase 1 ELISA Kit, C2H1orf130 ELISA Kit, legdh1 ELISA Kit, cb622 ELISA Kit, wu:fc33g09 ELISA Kit, wu:fc66a10 ELISA Kit, zgc:77186 ELISA Kit, glutamate dehydrogenase 1 ELISA Kit, glutamate dehydrogenase 1 S homeolog ELISA Kit, glutamate dehydrogenase 1, mitochondrial ELISA Kit, glutamate dehydrogenase 1b ELISA Kit, glutamate dehydrogenase ELISA Kit, non-compact myelin associated protein ELISA Kit, glutamate dehydrogenase 1a ELISA Kit, GLUD1 ELISA Kit, GDH1 ELISA Kit, glud1 ELISA Kit, glud1.S ELISA Kit, LOC693461 ELISA Kit, glud1b ELISA Kit, Glud1 ELISA Kit, HACJB3_RS00320 ELISA Kit, LOC100587725 ELISA Kit, NCMAP ELISA Kit, gdh1 ELISA Kit, glud1a ELISA Kit

Background:

Gene Name: Glutamate Dehydrogenase 1

Gene Aliases: GLUD1, Glutamate dehydrogenase 1, mitochondrial

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect